Inhibition of monocarboxylate transporter one in spinal-cord horn signif

Jian-hua He,^1,^* Ling Yu,^2,^* Zhi-yong Wang,¹ Qiang Wang,³ Jun-Li Cao,⁴ Lian-bing Gu¹

¹Department of Anesthesiology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research and also the Affiliated Cancer Hospital of Nanjing Medical College, Nanjing, People’s Republic of China ²Department of Ultrasound, Affiliated Hospital of Integrate Traditional Chinese and Western Medicine, Nanjing College of Traditional Chinese Medicine, Jiangsu Province Academy of Chinese Medicine, Nanjing,People’s Republic of China ³Department of Anesthesiology, Nanjing Meishan Hospital, Nanjing, People’s Republic of China ⁴Jiangsu Key Laboratory of Anesthesiology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, People’s Republic of China

*These authors contributed equally for this work

Correspondence: Lian-bing Gu
Department of Anesthesiology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research and also the Affiliated Cancer Hospital of Nanjing Medical College, Baiziting 42#, Nanjing, Jiangsu 210009, People’s Republic of China
Tel/fax +86 25 8328 4765
Email lbg6519@163.com

Purpose: Chronic inflammatory discomfort is a very common symptom in the clinic, and also the underlying mechanism isn’t being completely understood. Various research has shown that central and peripheral sensitization and synaptic plasticity could play crucial functions in chronic inflammatory discomfort. Furthermore, groups of monocarboxylate transporters (MCTs) are carefully associated with cellular metabolic process and synaptic plasticity, which is also reported that MCTs take part in chronic inflammatory discomfort. Nonetheless, there’s a possibility of the engaging role of MCT 1 is within chronic inflammatory discomfort, nevertheless its specific cellular level mechanism is not yet been investigated. Within our study, we hypothesized that MCT one in the spine dorsal horn plays a significant part in chronic inflammatory discomfort.
Methods: In experiment A, rats were been through nociceptive behavior testing at 1 d previous day and 1 d, 3 d, and seven d after finishing complete Freund’s adjuvant (CFA) injection. The examples collected for discovering MCT 1 by Western blotting. In experiment B, rats were at random split into four groups. Intrathecal injection of MCT 1 inhibitor and nociceptive behavior tests were performed 1 d previous day and 1 d, 3 d, 7 d, 14 d, and 21 d after CFA injection. MCT 1 and p-ERK levels in spine dorsal horn were measured by Western blotting, and GFAP in spine dorsal horn was detected by immunofluorescence.
Results: The expression of MCT one in the spine dorsal horn was elevated during chronic inflammatory discomfort in rats. The intrathecal injection of the MCT 1 inhibitor obviously reduced the expression of MCT 1 and GFAP within the spine dorsal horn, and also the behavior nociceptive responses were also attenuated. Meanwhile, the expression of p-ERK seemed to be decreased through the intrathecal injection of the MCT 1 inhibitor.
Conclusion: Our results indicate that MCT 1 most likely play a vital role in controlling chronic inflammatory discomfort and could influence the regulating synaptic plasticity via ERK within the spine dorsal horn of rats.

Keywords: chronic inflammatory discomfort, monocarboxylate transporter 1, spine dorsal horn, astrocyte, synaptic plasticity